Figure 7.

Expression of Aar inhibits biofilm formation in EAEC strain 042. A) The panel shows crystal violet biofilm formation assays, which monitor the ability of EAEC strains 042, 17–2, E36 and E42 to form biofilms on plastic microtitre plates, when grown in DMEM high glucose. B) The panel shows biofilm formation assays, monitoring the ability of EAEC 042 and EAEC 042 ΔaggR to form biofilms on plastic microtitre plates, when grown in DMEM high glucose. C) The panel shows the biofilm formed by EAEC strain 042 carrying either pBAD24, pBAD30, pBAD30/aar 042, pBAD30/aar* 042, pBAD30/aar E36 or pBAD30/aar* E36. Cells were grown in DMEM high glucose in presence (+) or absence (-) of 0.2% (w/v) arabinose. In all panels, the data displayed are representative experiments, with each value being an average of eight replicate samples