Fig 1. Development of CRISPR reagents targeting Ae. aegypti genes involved in flight.

(A) Workflow for performing embryo assays. Gene models and HRMA analysis following embryonic microinjection of CRISPR/Cas9 reagents with each group of sgRNAs for AeAct-4 (B), myo-fem (C), and Aeflightin (D). For (B-D), boxes represent exons, while cross-hatched areas represent the ORF of the corresponding mRNA. For each, red triangles indicate the locations of sgRNAs that were found to successfully cleave the DNA during embryo assay, and the blue and red boxes under the gene models indicate the approximate HRMA amplicon sizes. Melt curves are displayed for clusters of sgRNAs (indicated as “site” or “exon”), with sgRNA-injected (red) and non-injected (gray) samples.