Skip to main content
. 2020 Dec 18;16(12):e1008490. doi: 10.1371/journal.pcbi.1008490

Fig 7. Inhibition of CaMKII-Gβγ or CaN-Gβγ suppresses ISO-induced ERK1/2 activity.

Fig 7

(A) Effects of pretreatment for 30 min with five inhibitors including JAK-i (AG490), CaMKII-i (KN93), CaN-i (CsA), Gβγ-i (Gallein), and MEK-i (U0126) were examined on basal and ISO-induced ERK1/2 activity. (B) Upper panel shows western blotting of ERK1/2 phosphorylation after pretreatment with inhibitors in comparison with control (DMSO). Lower panel shows ERK1/2 phosphorylation after ISO stimulation with pretreatment with same inhibitors. *represents statistically significant change (p<0.05) in comparison with control (DMSO) for the upper panel, or with ISO in the lower panel. # represents significant change (p<0.05) compared with control (DMSO) in the lower panel. Data were collected from 3 independent experiments (Mean±SEM). (C) Immunofluorescence images show ISO-induced ERK1/2 phosphorylation and its suppression by MEK inhibitor in myocytes (alpha-actinin positive) and fibroblasts (alpha-actinin negative). The scale bar is 50 μm (D) Immunofluorescence imaging of ERK1/2 phosphorylation shows increase of ERK1/2 phosphorylation after CaMKII inhibition with KN93 and its suppression after pretreatment with Gβγ+CaMKII and Gβγ+CaN combined inhibitions. The scale bar is 200 μm.