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. 2020 Dec 24;11(1):1656–1673. doi: 10.1080/21505594.2020.1848108

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This work was authored as part of the Contributor’s official duties as an Employee of the United States Government and is therefore a work of the United States Government. In accordance with 17 U.S.C. 105, no copyright protection is available for such works under U.S. Law.

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Figure 1. — Evaluation of mycobacterial Isolates: (a) Difference in colony morphology of Mycobacterium bovis isolates within the BCG vaccinated patient with IFNγR1 deficiency compared to the vaccine Mycobacterium bovis BCG isolate. Colonies of the BCG strains at 3 weeks growth in Middlebrook 7H11 plate (100× magnification, Bar = 0.5 inch). (b) Quantification of colony-forming units (CFUs); from the lysate of peripheral mononuclear cells at d 2 of infection by the 3 strains. Data, mean ± SE (n = 6, **p < 0.01; *p < 0.05; two-tailed paired Student’s t-test). (c) Monocyte-derived macrophages were infected with GFP-transfected strain; at MOI of 2 for 2 d. Slides were made with DAPI staining for nucleic acid content. Representative infected cell of each strain, with also gray color as visible light showing bacteria inside of the limits of the cell (bar = 5 μm), with field showing more cells on smaller scale (bar = 100 μm). (d) Core genome phylogenetic analysis of 14 genomes; 3 BCG strains (BCG-vaccine, BCG-brain, and BCG-lung), 5 representative genomes of M. tuberculosis (H37Rv, CDC1551, F11, KZN 1435, and KZN 4207), and 6 M. bovis isolates (BAA_935, Tokyo 172, BCG1 Russia, BCG Pasteur 1173P2, BCG Danish 1331 Belgium, and BCG Denmark)