Figure 5.

Model illustrating the versatile capacity of Cep63 and Cep152 to phase-separate into morphologically distinct higher-order self-assemblies. Cep63 and Cep152 interact with each other and generate a heterotetrameric complex through the Cep63 (424–541) and Cep152 (1205–1272) regions [8]. In the presence of the intact hydrophobic motifs (one each from Cep63 and Cep152), the resulting complex can phase-separate into a condensate or an organized assembly, as evidenced by its ability to generate a densely deposited matrix-like aggregate that exhibits dynamic turnover and fusion and undergoes an internal rearrangement of its constituents. Our data demonstrate that the complex possesses a versatile capacity to generate inside-filled condensates (in the presence of a macromolecular crowder), hollow spherical assemblies (in a 3D space), or cylindrical assemblies (in a 2D surface) under different physicochemical and spatial cues. We propose that an unknown element(s) may dictate the Cep63•Cep152 complex to assume a cylindrical localization pattern around a centriole in vivo. A arrow denotes that a spherical condensate may convert to a vesicle-like, hollow spherical assembly, although its transition to a solid-like state cannot be ruled out