Figure 4.
Effects of mutation R498L on channel gating
A. Current-voltage relationships (ICa; mean ± SEM) of Cav1.38a-42 and Cav1.38a-11-42 vs R498L8a-11-42 recorded in parallel on the same day. Inset: Representative ICa traces of Cav1.38a-42, Cav1.38a-11-42 and R498L8a-11-42 upon depolarization to the Vmax. B. Normalized steady-state activation and inactivation curves of Cav1.38a-42 and Cav1.38a-11-42 vs R498L8a-11-42. Data are presented as mean ± SEM; for parameters and statistics see Table 3. C. Inactivation kinetics of Cav1.38a-42 and Cav1.38a-11-42 vs R498L8a-11-42 during a 5-s depolarization to Vmax with Ca2+ as a charge carrier showing no difference in inactivation kinetics between Cav1.38a-42 and Cav1.38a-11-42 vs R498L8a-11-42. Inset shows the first 2000 ms. Data are presented as mean ± SEM; for statistics see Table 4. Data were collected from >3 independent transfections.