Table 1.
Activation and inactivation parameters of Cav1.3 8b and 8a splice variants
| |
Activation |
Inactivation |
||||||
| α1-subunit | V0.5 (mV) |
Slope (mV) | Vrev (mV) |
n | V0.5 (mV) |
Slope (mV) | Non-inactivating (%) |
n |
| 8b 43S | -10.21± 0.62 |
8.51 ± 0.17 |
65.70 ± 0.88 |
25 | -35.71 ± 0.83 |
5.52 ± 0.20 |
17.84 ± 1.98 |
22 |
| 8b 11 43S | -13.40± 0.63*** |
7.60 ± 0.15*** |
64.75 ± 0.72 |
29 | -39.94 ± 0.97** |
4.94 ± 0.19 |
10.82 ± 0.78** |
21 |
| 8b 32 43S | -13.58 ± 0.55** |
8.36 ± 0.15 |
63.21 ± 0.84 |
16 | -41.53 ± 1.08*** |
5.21 ± 0.25 |
13.74 ± 2.23 |
12 |
| 8b 11 32 43S | -12.79 ± 0.58* |
8.33 ± 0.12 |
65.23 ± 0.91 |
19 | -39.54 ± 1.00* |
4.90 ± 0.16 |
10.80 ± 1.19** |
17 |
| 8a 43S | -10.99 ± 0.63 |
8.28 ± 0.16 |
65.67 ± 0.92 |
24 | -34.79 ± 0.90 |
4.67 ± 0.19 |
10.65 ± 0.87 |
15 |
| 8a 11 43S | -13.21 ± 0.63* |
7.6 ± 0.14** |
63.75 ± 0.91 |
19 | -37.99 ± 1.31 |
4.43 ± 0.18 |
14.23 ± 2.43 |
10 |
| 8a 32 43S | -13.03 ± 0.47 |
7.97 ± 0.13 |
63.80 ± 0.64 |
16 | -38.82 ± 0.92* |
5.04 ± 0.19 |
12.04 ± 1.28 |
13 |
| 8a 11 32 43S | -12.65 ± 0.76 |
8.23 ± 0.16 |
64.58 ± 0.81 |
17 | -37.15 ± 1.36 |
4.91 ± 0.10 |
15.36 ± 2.14 |
13 |
Parameters were obtained from fitting normalized activation curves (G/Gmax) or normalized steady-state inactivation curves (I/Icontrol). All values are presented as mean ± SEM and originate from >3 independent transfections. Statistics: one-way ANOVA followed by Bonferroni post-hoc test, *p < 0.05, **p < 0.01, ***p < 0.001 in comparison to 8b 43S or 8a 43S, respectively. n, number of experiments. V0.5, half-maximal activation/inactivation voltage; Vrev, reversal potential.