Table 3.
Activation and inactivation parameters of mutations R498L
| |
Activation |
Inactivation |
||||||
| α1 subunit | V0.5 (mV) |
Slope (mV) | Vrev (mV) |
n | V0.5 (mV) |
Slope (mV) | Non-inactivating (%) |
n |
| Cav1.38a-42 | 0.45 ± 0.90 |
9.51 ± 0.15 | 66.56 ± 0.86 | 44 | -17.35 ± 0.76 | 6.02 ± 0.18 | 19.56 ± 1.25 | 33 |
| Cav1.38a-11-42 | -1.31 ± 0.88 |
9.32 ± 0.20 | 63.75 ± 0.97 | 25 | -17.63 ± 0.81 | 6.01 ± 0.30 | 15.94 ± 1.76 | 17 |
| R498L8a-11-42 | 0.04 ± 1.03 |
9.28 ± 0.11 | 66.14 ± 1.04 | 30 | -16.48 ± 0.88 | 6.03 ± 0.37 | 17.79 ± 1.62 | 23 |
Parameters were obtained from fitting normalized activation curves (G/Gmax) or normalized steady-state inactivation curves (I/Icontrol). All values are presented as mean ± SEM and originate from >3 independent transfections. Statistics: One-way ANOVA, followed by Bonferroni multiple comparison posttest. n, number of experiments. V0.5, half-maximal activation/inactivation voltage; Vrev, reversal potential.