Table 1.
Summary of the top 20 CD160 scFv identified
| Clone | HCDR3 | VH gene | P3% | Obtained in conventional screening | ka (1/Ms) | kd (1/s) | KD (M) |
|---|---|---|---|---|---|---|---|
| 1 | ATHNYESTVFDY | IGHV5S13 | 38.6 | Yes | 1.79E+05 | <1E-06* | <5.58E-12* |
| 2 | VTHNYESTVFDY | IGHV5S13 | 19 | Yes | 2.49E+05 | 6.51E-05 | 2.62E-10 |
| 3 | ARGWTH | IGHV2-70 | 9.3 | Yes | 1.13E+06 | <1E-06* | <0.89E-12* |
| 4 | ATHNYESTIFDY | IGHV5S13 | 4.5 | No | 3.25E+05 | 3.90E-05 | 1.20E-10 |
| 5 | ARGMEA | IGHV2-70 | 4.2 | Yes | 1.65E+06 | 3.05E-02 | 1.84E-08 |
| 6 | ATHNYGSTIFDY | IGHV5S13 | 2.3 | Yes | 2.64E+05 | 1.43E-04 | 5.41E-10 |
| 7 | ARGLAY | IGHV2-70 | 2.2 | No | 1.06E+06 | 3.50E-04 | 3.30E-10 |
| 8 | ARGMDA | IGHV2-70 | 1.3 | No | 1.63E+06 | 8.04E-03 | 4.92E-09 |
| 9 | ATHNYESTVLIT | IGHV5S13 | 0.58 | No | 4.08E+05 | 2.11E-03 | 5.18E-09 |
| 10 | ARGFEY | IGHV2-70 | 0.48 | No | 2.75E+05 | 2.45E-03 | 8.91E-09 |
| 11 | ATIYYHDGSYYYAGWFAY | IGHV5S13 | 0.43 | No | 1.37E+05 | 1.25E-03 | 9.15E-09 |
| 12 | VTHNYESTIFDY | IGHV5S13 | 0.41 | No | 2.05E+05 | <1E-06* | <4.88E-12* |
| 13 | ARHGYGSLDY | IGHV5S13 | 0.31 | No | - | - | ND |
| 14 | TRDYGNYYFDY | IGHV6-8 | 0.24 | No | - | - | ND |
| 15 | VRGGSDY | IGHV5S13 | 0.23 | No | - | - | ND |
| 16 | ARGFEH | IGHV2-70 | 0.23 | No | 2.79E+05 | 2.43E-03 | 8.72E-09 |
| 17 | ATLPRY | IGHV5S13 | 0.18 | No | - | - | ND |
| 18 | ARHSGFAY | IGHV5-25 | 0.18 | No | - | - | ND |
| 19 | VAHNYESTVFDY | IGHV5S13 | 0.18 | No | 1.57E+05 | 8.33E-04 | 5.31E-09 |
| 20 | EHITTSPPFLIT | IGHV5S13 | 0.1 | No | 3.81E+05** | 2.66E-03** | 6.98E-09** |
Biophysical properties of the top 20 CD160 identified binders, by HCDR3 frequency, after 3 rounds of panning. (P3% = frequency of the HCDR3 in the round 3 of biopanning, ka = on-rate, kd = off-rate, KD = affinity). Clones which did not demonstrate binding in flow cytometry experiments are highlighted in grey (ND = not determined).
*kd outside instrument limits. kd value restricted to 1E-06.
**Outside instrument limits