Table 1.
Primer pairs spanning the BAC. Five primer pairs were used to amplify portions of the 245 kB of the RP23-90G21 BAC plus Cre recombinase, starting at the Adipoq promoter region (primer pair 1). FW: forward; RV: reverse
| Primer pair | Binding position | Direction | Binding position | Sequence (5ʹ→3ʹ) |
|---|---|---|---|---|
| 1 | 24 kb | RV | 23520 | AAGAGTTCTGGGTGTTGTTT |
| FW | 24117 | TTTGTCTGCAATGCTTTACC | ||
| 2 | 76 kb | RV | 75922 | TGGTTTGAGCAAGGTTATCA |
| FW | 76203 | CTCTTGCTGTAAGGGAACAT | ||
| 3 | 127 kb | RV | 127236 | GGAGTTGATTCGTCTCCTAC |
| FW | 127516 | TGGAAACGTCTAGGCTTATG | ||
| 4 | Cre | RV | 176000/480 | ATTACGTATATCCTGGCAGC |
| FW | 176001/907 | GGAGTTTCAATACCGGAGAT | ||
| 5 | 225 kb | RV | 223544 | GAGAAGTGGAGACTAGCATG |
| FW | 223747 | CTGTGACTTTGAGAACTTGC |