Figure 2.

Stable colonization of E. muris in the intestine of OligoMM¹² mice. a) Beta-diversity analysis shown as multidimensional scaling plot of generalized UniFrac distances (p-value <0.001; PERMANOVA). Bacterial composition in the gnotobiotic mice shown as b) relative abundances obtained by high-throughput 16S rRNA gene amplicon sequencing, and c) absolute gene copy numbers obtained by species-specific, 16S rRNA gene-targeted qPCR assays. d) Comparison of relative abundances between qPCR and 16S rRNA gene amplicon sequencing shown as ratios of respective relative abundances. For ratios below one, reciprocal values were calculated. Data are plotted as mean values ± SD. Significance is indicated as follows: one symbol, p < .05; two symbols, p < .01; three symbols, p < .001 (two-way ANOVA followed by Tukey test). Stars indicate differences due to E. muris (blue vs. gray). Hashtags indicate differences due to diet (mean values for LF diet-fed mice are shown again in gray in the plots for HF diet-fed mice)