Figure 4.

Effects of exercise training and detraining on gut permeability in SHR and WKY rats. The mRNA levels of tight junction proteins Tjp1 (a), Ocln (b) and Cldn4 (c) in small intestine (ileum) in all experimental groups. (d) Representative immunohistochemistry images showing Tjp1-, Ocln- and Cldn4-positive cells in the ileum in SHR and WKY rats. (e) A representative immunoblot; and (f-h) densitometric analysis of protein expression of Tjp1, Ocln and Cldn4 in the ileum in SHR and WKY rats. (i) Measurement of intestinal FABP level in the plasma in SHR and WKY rats. n = 8–10 rats per group. Data are presented as mean ± SEM. *P< .05; **P< .01; ***P< .001; ****P< .0001 using two-way ANOVA with a Tukey’s post-hoc test