Table 1.
Rat primers used for real-time RT-PCR
| Rat genes | Forward Primer (5′-3′) | Reverse Primer (5′-3′) |
|---|---|---|
| TNF-α | GTCGTAGCAAACCACCAAGC | TGTGGGTGAGGAGCACATAG |
| IL-1β | GCAATGGTCGGGACATAGTT | AGACCTGACTTGGCAGAGA |
| IL-6 | TCTGGTCTTCTGGAGTTCCGT | GCATTGGAAGTTGGGGTAGGA |
| TLR2 | GCACTTGAGCGAGTCTGCTTTC | GAACAAATAGAACTGGGGGATGTG |
| TLR4 | GGCTGTGGAGACAAAAATGACCTC | AGGCTTGGGCTTGAATGGAGTC |
| Tjp1 | AGCGAAGCCACCTGAAGATA | GATGGCCAGCAGGAATATGT |
| Ocln | CTGTCTATGCTCGTCATCG | CATTCCCGATCTAATGACGC |
| Cldn4 | CGAGCCCTGATGGTCATCAG | CGGAGTACTTGGCGGAGTAG |
| HMGB1 | CTAGCCCTGTCCTGGTGGTATT | CCAATTTACAACCCCCAGACTGT |
| TH | CGAGCTGTGAAGGTGTTTGA | GTACACCTGGTCCGAGAAGC |
| GAPDH | AGACAGCCGCATCTTCTTGT | CTTGCCGTGGGTAGAGTCAT |
TNF-α, tumor necrosis factor-alpha; IL, interleukin; TLR, toll-like receptor; Tjp1, tight junction protein 1; Ocln, occluding; Cldn4, claudin 4; HMGB1, high mobility box 1; TH, tyrosine hydroxylase; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase.