Figure 1.
Effect of Cav3.3-blocking peptide on mechanical allodynia in FRICT-ION mice. A Cav3.3-blocking peptide, TAT-C3P, was administered to (A) male and (B) female mice via intraperitoneal injection at a dose of 10 mg/kg and monitored every hour for up to 5 h post-injection. For control experiments TAT-C3D was used, which does not block Cav3.3 channels. (C) Effectiveness of 10 mg/kg Cav3.3-blocking peptide at peak anti-allodynic effect (4 hrs post-injection) is compared versus naïve, TAT-C3D control, and versus male and female mice * p < 0.05 and ** p < 0.01 compared to FRIC+TAT-C3D, **** p < 0.0001 compared to FRIC+TAT-C3D, ++ p < 0.01 compared to males with FRIC+TAT-C3D. Lack of error in naïve behavioral measurements is because of a ceiling effect for mechanical threshold in these mice