Table 1.
RNAseq analysis showing up- and down-regulated ion channel gene expression in TG isolated from FRICT-ION mice. The expression of all Cav3 subtypes, Cacna1g, Cacna1h, Cacna1i is indicated. Cacna1i expression increase is statistically significantly (p < 0.05, paired t-test), whereas that of Cacna1g and Cacna1h are not altered (p > 0.05, paired t-test) compared to naïve controls. Expression of housekeeping gene Gapdh is not significantly changed compared to controls (p > 0.05, paired t-test). TG were isolated at 10 weeks post-injury. This analysis is based on RNA obtained from whole TG tissue of three FRICT-ION and three naïve male mice. Percentage change with corresponding p-values is indicated to three significant figures
| Gene symbol | Name | % change | p-value |
|---|---|---|---|
| Upregulated ion channel genes | |||
| Cacna1g | calcium channel; voltage-dependent; T type; alpha 1 G subunit | 35.6% | 0.358 |
| Cacna1h | calcium channel; voltage-dependent; T type; alpha 1 H subunit | 10.9% | 0.160 |
| Cacna1i | calcium channel; voltage-dependent; alpha 1I subunit | 39.8% | 0.0276 |
| Gapdh | glyceraldehyde-3-phosphate dehydrogenase | 3.47% | 0.590 |
| Kcnh6 | potassium voltage-gated channel; subfamily H (eag-related); member 6 | 20.3% | 0.0449 |
| Clic4 | chloride intracellular channel 4 (mitochondrial) | 25.1% | 0.0208 |
| Kcnj10 | potassium inwardly-rectifying channel; subfamily J; member 10 | 37.0% | 0.000219 |
| Hvcn1 | hydrogen voltage-gated channel 1 | 60.4% | 0.0370 |
| Kcnj16 | potassium inwardly-rectifying channel; subfamily J; member 16 | 81.5% | 0.0102 |
| Downregulated ion channel genes | |||
| Kcnb2 | potassium voltage gated channel; Shab-related subfamily; member 2 | −44.2% | 0.0270 |
| Kcnv1 | potassium channel; subfamily V; member 1 | −40.0% | 0.0112 |
| Kcnh5 | potassium voltage-gated channel; subfamily H (eag-related); member 5 | −35.3% | 0.0194 |
| Kcnh7 | potassium voltage-gated channel; subfamily H (eag-related); member 7 | −34.4% | 0.0302 |
| Kcnmb2 | potassium large conductance calcium-activated channel; subfamily M; beta member 2 | −32.0% | 0.0468 |
| Scn1a | sodium channel; voltage-gated; type I; alpha | −25.2% | 0.00392 |
| Kcnq3 | potassium voltage-gated channel; subfamily Q; member 3 | −21.2% | 0.0321 |
| Kcna2 | potassium voltage-gated channel; shaker-related subfamily; member 2 | −18.3% | 0.0341 |
| Scn9a | sodium channel; voltage-gated; type IX; alpha | −16.1% | 0.0414 |
| Nalcn | sodium leak channel; nonselective | −14.4% | 0.0172 |
| Trpm7 | transient receptor potential cation channel; subfamily M; member 7 | −13.6% | 0.0171 |