Table 1.
Strain characteristic, MLST, capsular locus type, resistome and virulome of UCO-494 and UCO-495
| Phenotype | UCO-494 hmKp |
UCO-495 hmKv |
|---|---|---|
| Origin | Blood | Catheter |
| year | 2012 | 2012 |
| String test | + | + |
| ST | 1161 | 173 |
| K-locus* | KL19 | KL25 |
| ybt | ybt14 ICEKp5 | - |
| ybST | 327–1LV | - |
| O-locus | O1v2 | - |
| ESBL combined disc test | + | + |
| WGS data | ||
| Contig number | 462 | 226 |
| Genome size (bp) | 6,400,426 | 5,982,509 |
| GC% | 56,4% | 56,1% |
| CDS; pseudogenes; tRNA | 6668;203;95 | 5968;169;79 |
| Resistance profile | ERT, CIP, LEV AMK, KAN, GEN, TOB, AMP, CTX, CAZ, AMC | TET, STX, W, CPD, CRO, AMK, KAN, GEN, TOB, AMP, CTX, CAZ, FEP, AMC |
| MIC colistin | 8 μg/mL | 16 μg/mL |
| Resistome | ||
| Antibiotic resistance genes | sul1; sul2; arr-2; dfrA12; aadA1; aadA2; aac(6`)-Ib; aac(6`)-Ib-cr; oqxA/B; qnrB19; qnrB4; blaCTX-M-1; blaDHA-1; blaOXA-10; blaOXA-9; blaSHV-187; gyrA83L; gyrA87Y; parC80I | blaLEN-25; blaSHV-12; blaTEM-1B; oqxA; oqxB; aph(3”)-Ia; aph(6)-Id; aph(3”)-Ib; tet(D) |
| colistin mutation gen | PmrB: Gly256Arg (G766C) | PmrA: Thr146Ala (A436G); PmrB: Ser170Ala (G508T); PhoQ: Asp152Glu (T456G) |
| Heavy-metal resistance genes | arsenic (arsCDBAH); magnesium/cobalt/nickel/manganese (corA); glyphosate (phnMLKJI); quaternary ammonium (emrD – qacE∆1) | arsenic (arsBCRD), cobalt/manganese (corC), cobalt/magnesium (mgtA), magnesium/cobalt/nickel/manganese (corA): tellurium resistance gen (terW and terZCD) |
| Virulome | ||
| Virulence genes | Enterobactin (entB; entF; ycfH; entD), urea(ureA), alantoin (allS), aerobactin (iutA), fimbria type 1 (fimABCDFEGH), fimbria type 3 (mrkABCDF), yersiniabactin (irp1; irp2; fyuA; ybtAES), colicin V (cvpA; cvaA), biofilm (treC; sugE), ECP (ecpABCDE) | Urea (ureA), alantoin (allS), aerobactin (iutA), fimbria type 1 (fimABCDFEGH), fimbria type 3 (mrkABCDF), colicin V (cvpA; cvaA), biofilm (treC; sugE), ECP (ecpABCDE), KFU (kfuABC) |
| Plasmids | ColRNAI; IncA/C2; IncFIB (3); IncFII | IncFIB; IncFII; IncHI2; IncHI2A |
hmKP, hypermucoviscous Klebsiella pneumoniae; hvKP, hypervirulent Klebsiella pneumoniae; *Capsular polisacharide concentration in OD650nm 2.0. Significative difference with p-value equal to 0.0001 in t test. ERT, ertapenem; CIP, ciprofloxacin; LEV, levofloxacin; AMK, amikacin; KAN, kanamycin; GEN, gentamicin; TOB, tobramycin; AMP, ampicillin; CTX, cefotaxime; CAZ, ceftazidime; FEP, cefepime; ; CPD, cefpodoxime; CRO, ceftriaxone; AMC, amoxicillin-clavulanic acid; W, trimethoprim; TET, tetracycline; SXT, sulphamethoxazole-trimethoprim .