Figure 1.

Thi-hyd reduced the viability and migration of TNBC cells. (a) The structure of Thi-hyd. (b) Cell viability was reduced by Thi-hyd in a dose-dependent manner. The cell viability was detected by CCK-8 assay after 48 hours or 72 hours of treatment with indicated dose of Thi-hyd. Results were expressed as average ± S.D. (n = 6, one way ANOVA, *p < 0.05, ***p < 0.001) (c) Cell colony formation was inhibited by Thi-hyd in a dose-dependent manner. The colonies of 4T1 and MAD-MB-231 cells were stained with crystal violet following 6–8 days treatment of different dose of Thi-hyd (0μM, 5 μM, 10 μM, 20 μM, 40 μM). (d) Thi-hyd inhibited the phosphorylation of AKT instead of ERK1/2 in TNBC cells. Cells were treated with various concentrations of Thi-hyd for 24 hours. The phosphorylation of the AKT and ERK1/2 was determined by western blotting. GAPDH was used as internal control. (e) Thi-hyd inhibited the migration of 4T1 cells. 4T1 cells were seeded on the up chamber of transwells and treated with various concentrations of Thi-hyd for 24 hours. Then the cells were dried and stained with crystal violet. The representative picture from 3 replicates in each group was shown in this figure. Scale bar, 200 μm