Figure 1.

Fab1 identification and validation. (a) Domain analysis of Fab1 in different species. (b) Relative fab1 mRNA levels under different growth conditions. Data are presented as means ±S.D. and represent the results of three independent experiments. Statistically significant differences are indicated: *p < 0.05. (c) Validation of fab1 transcript quantification via northern blotting. (d) PCR validation of fab1 fragment clones by 3’RACE. The second round of PCR was performed with the gene-specific forward primers and the RACE adaptor primers. The PCR products were analyzed by electrophoresis. Fragment1 and Fragment2 are two independent biological replicates. (e) Sequencing validation of amplified fragment from fab1 by 3’RACE. The amplified fragment by 3’RACE included the splice site and ploy (A) of fab1.