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. 2020 Dec 28;2020:8821181. doi: 10.1155/2020/8821181

Figure 1.

Figure 1

Development of Aβ1-42 detection. (a) Indirect ELISA calibration curves for Aβ1-42 using 1 : 1,000 (v/v) dilution of alpaca antiserum. (b) Optimization of coating antigen using indirect competitive ELISA. The percent binding showed a competition percentage of 6 concentrations of competitive Aβ1-42 (62, 125, 250, 500, 1,000, and 2,000 ng/ml) to a 1 : 1,000 (v/v) dilution of alpaca antiserum at 3 concentrations of coating antigen (■ = 2,000, ▲ = 3,000, and ▼ = 4,000 ng/ml of Aβ1-42 in coating buffer). (c) ic-competitive ELISA calibration curves for Aβ1-42 using a 1 : 1000 (v/v) dilution of alpaca antiserum. The calibration curve was obtained using analyte standards prepared in PBS buffer. (d) ic-competitive ELISA calibration curves obtained by OD.