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. 2020 Sep 24;9(1):1824643. doi: 10.1080/2162402X.2020.1824643

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© 2020 The Author(s). Published with license by Taylor & Francis Group, LLC.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — A2AR knock-out enhanced the anti-tumor function of CAR-T cells in vitro. (a) Specific lysis of AKO and P4 cells after incubation with CRL5826 at 1:1 E:T ratio and 0.5:1 E:T ratio with 3 d in the presence of 0, 0.2, 1, 5, and 10 µM CADO. (b) Cytokine IFN-γ and IL-2 production by AKO and P4 cells cocultured 3 d with CRL5826 at 0.5:1 E/T ratio in the presence of 0 and 5 µM CADO. (c) Expression changes of IFNG and GZMB in AKO and P4 cells after incubation with CRL5826 at 0.5:1 E:T ratio with 3 d in the presence of 0 and 5 µM CADO confirmed by qPCR. *P < .05; ***P < .001; ****P < .0001 were determined by two-way ANOVA test. Data were represented as mean ± s.d. of three technical replications per assay. The assays were repeated three times in (a) and two times in (b–c).