Figure 2.
Impaired germination rates in MYB75pr:MYB75T131A and MYB75pr:MYB75T131E transgenic plants. Seeds from homozygous T3 plants were plated on ½ MS agar medium along with myb75− and Nossen WT controls. After vernalization, the plates were placed under light fluence of ~30 µmol m-2 s − 1 and a 16 h light/8 h dark photoperiod, at 21°C. Germination rate was scored ten days after transferring the plates into light. Percentage germination was determined for each line by counting the total number of seeds and the total number of germinated plants. Percentages of germinated plants were averaged for each genotype, error bars represent ±SD. Germination rates were 26.8% (SD = 9.99) for MYB75pr:MYB75T131A plants and 62% (SD = 12.62) for MYB75pr:MYB75T131E. These data indicate that MYB75pr:MYB75T131A plants have a significantly lower germination rate than either Nossen WT, myb75− or MYB75pr:MYB75T131E transgenic lines. T test <0.05