Figure 1.

NK cell activation by immobilized anti-NKG2D ISVDs. Bacterial supernatants containing soluble ISVDs were added onto 96-well microplates pre-coated with a 1:1 mix of either anti-HA tag mAb and mIgG1 (gray bars) or anti-HA tag mAb and anti-2B4 mAb (dark gray bars). IL-2 pre-stimulated NK cells were incubated onto plates for 2 h, harvested and the percentage of CD107a-positive cells was quantified by flow cytometry. PMA/Ionomycin was used as positive control (white bar). Control conditions with anti-CD16 ISVD, anti-CD16, anti-2B4, anti-NKG2D mAbs were included. Each bar represents the mean value ± SEM of 3 independent assays (different donors). One-way analysis of variance with repeated measures and Bonferroni-Holm adjustment were used to compare each compound with irrelevant ISVD. * P < .05, ** p < .001, ***P < .0001