Figure 8.

Effect of soluble NKG2D ligand on redirected NK cell lysis mediated by bsFabs. CFSE-stained BT-474 cells were incubated for 2 h with unstimulated human NK cells (E/T:6/1) and 40 nM bsFabs in the presence (dark gray bar) or absence (light gray bar) of 123 nM human His-tagged MICA. TO-PRO®-3 was used as dead cell indicator. BT-474 cell killing was quantified by flow cytometry as the percentage of double positive CFSE/TO-PRO-3 cells. Each bar represents the mean value ± SEM of 4 independent experiments. The p values were calculated with one-tailed Student’s t-test for bsFab in the presence or absence of soluble MICA. * P < .05