Figure 1.

Construction of TT1-E7E6. Schematic view of (a) a LCMV wt particle (left) and its genome (right). The ambisense RNA genome encodes 4 viral proteins: GP (glycoprotein), NP (nucleoprotein), L (RNA-directed RNA polymerase) and Z (RING finger protein Z). (b) The engineered TT1-E7E6 vectors contain artificially duplicated S-segments, encoding either GP or NP but not both, and a fusion protein of HPV16 E7E6, with 5 implemented amino acid mutations as indicated by the asterisks (*), as well as the L-segment.