Table 1.

Surface antigen expression of lymphoma cell lines. Raji, Daudi and JeKo-1 cell lines were screened for target antigen expression using flow cytometry. Lymphoma cells were co-incubated with primary biotinylated antibodies for 15 min at 4°C. Antigen expression was detected using a secondary PE-coupled anti-biotin antibody. Mean positivity and mean fluorescence index (MFI) values ± SD were calculated using staining with the secondary antibody alone as a negative control, n = 3.

	Raji		Daudi		JeKo-1
	Positive cells	MFI	Positive cells	MFI	Positive cells	MFI
CD19	99.97 ± 0.06%	107.72 ± 22.13	99.90 ± 0.00%	74.08 ± 4.50	97.93 ± 1.90%	21.93 ± 3.51
CD20	95.8 ± 3.99%	44.57 ± 15.78	98.5 ± 0.69%	85.85 ± 40.91	97.07 ± 3.35%	40.42 ± 19.28
CD22	99.1 ± 0.35%	47.90 ± 6.80	98.60 ± 0.69%	77.32 ± 26.56	24.07 ± 3.48%	1.80 ± 0.09
CD30	2.77 ± 0.92%	1.10 ± 0.10	0.57 ± 0.21%	1.04 ± 0.01	1.49 ± 0.49%	1.07 ± 0.02
CD38	98.43 ± 1.59%	57.50 ± 20.20	95.07 ± 1.16%	191.70 ± 11.45	81.07 ± 2.35%	17.69 ± 4.47
CD70	98.83 ± 1.68%	92.02 ± 23.64	41.97 ± 8.38%	2.93 ± 0.52	98.47 ± 0.29%	35.11 ± 8.53
CD79b	66.23 ± 9.82%	5.24 ± 2.08	98.30 ± 1.01%	76.67 ± 24.13	96.30 ± 0.56%	27.66 ± 0.59