Table 3.
Functional categories of various sulfenylated proteins identified from Arabidopsis and Medicago sulfenome
| Functional categorization | Protein | Subcellular location/number of cysteines | Method of detection | References |
|---|---|---|---|---|
| Arabidopsis sulfenome | Functional categorization of major types of sulphenylated proteins identified as components of Arabidopsis and Medicago sulfenome in response to oxidative stress (H2O2]. The sulfenome analysis was performed by Dyn-2 chemical probe and YAP1-c genetic probe approaches [87,113]. | |||
| Type 1- Protein degradation | OVARIAN TUMOR DOMAIN (OTU)-CONTAINING DUB (DEUBIQUITILATING ENZYME) 5 |
Cytosol (3) | DYn-2 chemical probe method and TAIR | 113 |
| Alpha/beta-Hydrolases superfamily protein | Cytosol, chloroplast [1) | DYn-2 chemical probe method and TAIR | 157,158 | |
| Peptidase M1 family protein | Cytosol, chloroplast [7) | DYn-2 chemical probe method and TAIR | 87 | |
| Zn-dependent exopeptidases superfamily protein | Cytosol, chloroplast [11] | DYn-2 chemical probe method and TAIR | 159 | |
| Cytosol aminopeptidase family protein | Cytosol, chloroplast (5] | DYn-2 chemical probe method and TAIR | 80 | |
| UBIQUITIN-ACTIVATING ENZYME 1 | Cytosol, nucleus and plasma membrane (18) | DYn-2 chemical probe method and TAIR | 113 | |
| COP9 SIGNALOSOME 5A | Cytosol, nucleus [2) | DYn-2 chemical probe method and TAIR | 87 | |
| 20S PROTEASOME BETA SUBUNIT G1 and 26S PROTEASOME REGULATORY COMPLEX | Cytosol (1] and chloroplast (8) | DYn-2 chemical probe method and TAIR | 80 | |
| CALRETICULIN 1A, 1B | Endoplasmic reticulum | DYn-2 chemical probe method and TAIR | 12 | |
| Ubiquitin-specific protease 12 (UBP12] and Ubiquitin-specific protease 13 (UBP13) | Cytoplasm [11) | YAP1-c | 87 | |
| Type 2- Primary metabolism | Pyruvate kinase family protein | Cytoplasm, plasma membrane (11] | 80 | |
| CYTOSOLIC-NAD-DEPENDENT MALATE DEHYDROGENASE 2 |
Cytoplasm, plasma membrane [6) | 160 | ||
| PHOSPHOENOLPYRUVATE CARBOXYKINASE | Cytoplasm, nucleus (12] | 113 | ||
| ARABINOSE KINASE | Cytoplasm, plasma membrane (22) | |||
| NADP-DEPENDENT MALATE DEHYDROGENASE |
Cytoplasm, apoplast [9) | 161,162 | ||
| S-FORMYLGLUTATHIONE HYDROLASE | Cytoplasm, apoplast [5] | 159 | ||
| GLYOXALASE I HOMOLOG | Cytoplasm, peroxisome, plastids, plasma membrane (1] | 113 | ||
| ACONITASE 1 | Cytoplasm, mitochondria plastids, plasma membrane, vacuoles (13) | |||
| IAA-CONJUGATE-RESISTANT 4 | Mitochondria [5) | 160 | ||
| ATP SYNTHASE ALPHA/BETA FAMILY | Mitochondria [3] | 12 | ||
| SUCCINYL-COA LIGASE, ALPHA SUBUNIT | Mitochondria, cell wall [8] | 158 | ||
| CYTOCHROME C-1 | Mitochondria, cytosol (2] | 113 | ||
| LONG-CHAIN ACYL-COA SYNTHETASE 4/ AMP-DEPENDENT SYNTHETASE AND LIGASE FAMILY PROTEIN |
Golgi apparatus, nucleus, plasma membrane (13) | |||
| 6-phosphogluconate dehydrogenase family | Plastid, mitochondria (6) | |||
| HEAT SHOCK COGNATE PROTEIN 70–1 | Plastid (4) | |||
| PLASTID ISOFORM TRIOSE PHOSPHATE ISOMERASE, |
Plastid [2) | 159 | ||
| Vacuolar ATP synthase subunit A (VHA-A] | Vacuolar membrane [6) | YAP1-c | 87 | |
| NADP-malic enzyme 2 (NADP-ME2] | Cytoplasm (7) | YAP1-c | ||
| Hormone homeostasis | RECEPTOR FOR ACTIVATED C KINASE 1B | Cytoplasm, ribosome and nucleus (2) | ||
| ACC OXIDASE 2 | Cytoplasm, endoplasmic reticulum | 113 | ||
| Amino acid metabolism | ARABIDOPSIS CYSTEINE SYNTHASE 1 | Plastid (5) | ||
| O-ACETYLSERINE (THIOL) LYASE ISOFORM C | Chloroplast, mitochondria (3) | |||
| PHOSPHOSERINE AMINOTRANSFERASE | Plastid (8) | |||
| ANTHRANILATE SYNTHASE 2 | Plastid (4) | |||
| ASPARTATE AMINOTRANSFERASE | Plastid (6) | |||
| VALINE-TOLERANT 1 | Plastid, cytosol (2) | |||
| SERINE-HYDROXYMETHYLTRANSFERASE 3 | Plastid (7) | |||
| Pyridoxine biosynthesis 1.3 (PDX1.3) | Plastid [7) | YAP1-c | 87 | |
| Redox-activity | STROMAL ASCORBATE PEROXIDASE | Plastid [4] | 163 | |
| MONODEHYDROASCORBATE REDUCTASE 6 | Plastid (5] | 113 | ||
| DIHYDROLIPOYL DEHYDROGENASES | Plastid [9) |
87 113 113 113 113 |
||
| GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE A SUBUNIT |
Plastid (5) | |||
| ELECTRON TRANSFER FLAVOPROTEIN BETA PHOSPHATE TRANSPORTER 3 (MPT3) |
Mitochondria (3) | |||
| THIOREDOXIN-DEPENDENT PEROXIDASE 1 | Cytosol, chloroplast, plasmamembrane | |||
| ATNRX1, NRX1, NUCLEOREDOXIN 1/DC1 domain-containing protein |
Cytosol (12) | |||
| AMINO ACID DEHYDROGENASE FAMILY | Cytosol (6) | |||
| Protein folding and transport | IMPORTIN ALPHA ISOFORM 1 | Cytosol, nuclear envelope, nulcleus (11) | ||
| HEAT SHOCK PROTEIN 81–3 | Cytosol, golgi, plasma membrane (5) | |||
| HEAT SHOCK COGNATE PROTEIN 70–1 | Cytosol, golgi, plasma membrane (7) | |||
| TCP-1/cpn60 chaperonin family protein | Cytosol, golgi, plasma membrane (9) | |||
| CHAPERONIN-60BETA3 | Cytoplasm, nucleus (6) | |||
| ERBB-3 BINDING PROTEIN 1 | Nucleus, plasma membrane (6) | |||
| LUMINAL BINDING PROTEIN | Endoplasmic reticulum (5) | |||
| γ-Glutamyl peptidase 3 (GGP3) | Cytoplasm (8) | YAP1-c | ||
| RNA-binding translation | ATP binding*leucine-tRNA ligases*aminoacyltRNA ligases*nucleotide binding*ATP |
Cytosol (20) | ||
| EUKARYOTIC TRANSLATION INITIATION FACTOR 3B-2 |
Cytosol, nucleus (3) | |||
| Nucleic acid-binding, OB-fold-like protein | Cytosol, plasma membrane (4) | |||
| CYCLOPHILIN 40 | Cytosol (7) | |||
| TRANSLATION INITIATION FACTOR 3 SUBUNIT H1 | Cytosol (7) | |||
| EUKARYOTIC TRANSLATION INITIATION FACTOR ISOFORM 4G1 |
Cytosol, nucleus (7) | |||
| Signal transduction | PEROXISOMAL 3-KETOACYL-COA THIOLASE 3 | Peroxysome (9) | ||
| CO-FACTOR FOR NITRATE REDUCTASE AND XANTHINE DEHYDROGENASE | Cytoplasm (9) | |||
| PHOSPHOLIPASE D ALPHA 1 | Cytoplasm (8) | |||
| INOSITOL 1,3,4-TRISPHOSPHATE 5/6-KINASE 4 (ITPK4) |
Cytosol, nucleus [9) | 87 | ||
| Map kinase 2, 4 and 7 (MAPK] | Cytosol [8) | YAP1-C | 87 | |