Figure 1.

Rab8a localises to Ruffles and Macropinosomes in LPS stimulated Mouse Macrophages. (a) Fluorescence microscopy images of fixed LPS-treated (30 mins) RAW 264.7 cells transiently overexpressing td-Tomato-Rab8a and stained with Alexa488-phalloidin. (b) Live cell confocal spinning disc images of LPS-treated RAW 264.7 cells transiently transfected with GFP-Rab8a showing enrichment of Rab8a on macropinosomes and subsequent internalisation via tubules. Movies were taken over 15 mins at 5 sec intervals. Scale bars, 10 µm