Fig. 5.

Lin28B elevates TET3 expression by repressing let‐7i. (A) Bioinformatics prediction of let‐7i binding sites in Lin28B 3′UTR or TET3 3′UTR sequence using TargetScan was shown. (B) Lin28B knockdown resulted in significant upregulation of let‐7i in PANC1. (C) The protein and mRNA levels of Lin28B and TET3 were measured in PANC1 transfected with miR‐NC or let‐7i. (D) The protein and mRNA levels of Lin28B and TET3 were assessed in PANC1 transfected with vector, Flag‐Lin28B, or Flag‐Lin28B and let‐7i. (E) The relative luciferase activities were detected by transfecting Lin28B wt or Lin28B mut and miR‐NC or let‐7i into PANC1. (F) Dual‐luciferase assays showed diminished luciferase activity when co‐transfected with TET3 wt, and let‐7i occurred in PANC1. Results represented the average of triplicate samples from three independent experiments. Each error bar represents the standard error of the mean. Statistical analysis was performed using the unpaired Student’s t‐test.