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. 2020 Nov 20;20:218–226. doi: 10.1016/j.omtm.2020.11.010

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© 2020 The Author(s)

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Immunohistochemical analysis of the distal colon of DSS-administered (8 days), psiTNF-treated (4 days) animals

(A) Distal colon of DSS-administered (8 days), psiTNF-treated (4 days) animals (mucosa, submucosa): psiTNF plasmid (GFP), Iba1⁺ mucosal macrophages (arrows), and epithelial cells (arrowheads). (B–D) Distal colon of DSS-administered (8 days), psiTNF-treated (4 days) animals (lymphatic aggregate in mucosa): psiTNF plasmid (GFP); GFP/IBA double-positive macrophages (arrowheads). (C and D) High-magnification images: Iba1⁺GFP⁺ (arrowheads) and Iba1⁺GFP⁻ macrophages (arrow in D). (E and F) Distal colon of DSS-administered (8 days), psiTNF-treated (4 days) animals (lymphatic aggregate in mucosa): psiTNF plasmid (GFP); GFP is expressed in CD45⁺Iba1⁺ mucosal macrophages (arrowheads in E); only pycnotic and ramified CD45/Iba1 double-positive macrophages express GFP, whereas CD45⁺Iba1⁻ lymphocytes of round morphology show no plasmid incorporation (F). (G–I) Distal colon of DSS-administered (8 days), psiTNF-treated (4 days) animals (mucosa); psiTNF plasmid (GFP); F4/80⁺ mucosal macrophages and epithelial cells show strong TNF production (G). (H and I) Expression of TNF and GFP of F4/80⁺ mucosal macrophages in consecutive sections.