Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Nov 16;473(1):79–93. doi: 10.1007/s00424-020-02491-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 1 — Characterization of NCC-cre-ERT2 mice. A Schematic representation of insertion of the tamoxifen-inducible creERT2 in the untranslated region (UTR) of the last exon of the Slc12a3/NCC gene. B Immunostaining for NCC, cre, and calbindin D28K (CB28) on consecutive cryosections from NCC-cre-ERT2 mice. Tamoxifen induces strong nuclear localization of the cre recombinase in NCC-positive DCT1 (1) and in DCT2 (2) with strong NCC and CB28-immunostaining. C Overviews on kidneys from NCC-cre-ERT2/tomato-flox mice (upper panel) show a strong tomato-related fluorescence in a kidney of tamoxifen-induced but not in the kidney of an uninduced mouse. High-power magnification (lower panel) demonstrate that the tomato-related fluorescence is strictly limited to the DCT (D) and is not found in the preceding NKCC2-positive TAL (T) and the downstream localized AQP2-positive CNT (CN). The segment transitions are indicated by double-headed arrows. Two pairs of consecutive cryosections. D and E Immunoblots and densitometry reveal that the creERT2 transgene does not interfere with NCC abundance and phosphorylation. Data are mean ± SEM, n = 3