Fig. 3.
DCT morphology and fractional cortical volume of DCT in Prox-1DCTCtrl versus Prox-1DCTKO mice at age 10 days and 6 weeks. A NCC protein expression (immunostaining on paraffin sections) in kidneys of 10-day-old pups (upper panel) and 6-week-old (lower panel) Prox-1DCTCtrl versus Prox-1DCTKO mice. Cre recombination and deletion of Prox-1 in the DCT was induced immediately after birth. B No difference in the fractional cortical volume (%) of whole DCT in Prox-1DCTCtrl versus Prox-1DCTKO mice was observed upon deletion of Prox-1, neither in pups nor in 6-week-old mice. DCTs were identified due to NCC immunostaining. Values are means ± SEM. n = 4 (10 days, ctrl + ko), n = 6 (6 weeks, ctrl), n = 5 (6 weeks, ko). C Distal convoluted tubules (D) in the renal cortex of Prox-1DCTCtrl and Prox-1DCTKO mice reveal a normal DCT morphology (adult kidneys) in both genotypes, epon semi-thin sections