Table 1.
Metabolic parameters, plasma and urine electrolyte concentrations and plasma aldosterone, 11-deoxycorticosterone, and corticosterone in Prox-1DCTKO mice
| Prox1DCTCtrl | Prox1DCTKO | |
|---|---|---|
| Age (days) | 90 ± 1 | 100 ± 3 |
| Body weight (g) | 31.3 ± 0.9 | 31.6 ± 1.0 |
| Food intake (g/day) | 7.3 ± 0.6 | 5.9 ± 0.3 |
| Water intake (ml/day) | 2.6 ± 0.6 | 3.3 ± 0.8 |
| Urine volume (ml/day) | 1.2 ± 0.3 | 1.1 ± 0.3 |
| Plasma Na+ (mmol/l) | 149.4 ± 0.6 | 150.9 ± 1.3 |
| Plasma K+ (mmol/l) | 4.0 ± 0.1 | 4.1 ± 0.2 |
| Plasma Ca2 + − (mmol/l) | 1.26 ± 0.03 | 1.25 ± 0.03 |
| Plasma Mg2+ (mmol/l) | 0.85 ± 0.03* | 0.76 ± 0.03* |
| Plasma HCO3− (mmol/l) | 19.3 ± 0.5 | 20.0 ± 0.5 |
| Plasma PO43−(mmol/l) | 1.61 ± 0.12 | 1.83 ± 0.15 |
| Plasma aldosterone (nM) | 0.32 ± 0.04 | 0.37 ± 0.09 |
| Plasma 11-Deoxycorticosterone (nM) | 1.38 ± 0.36 | 0.98 ± 0.37 |
| Plasma corticosterone (nM) | 107.7 ± 20.8 | 92.9 ± 19.0 |
| Urine Na+/urine creat. (mmol/mmol) | 40.8 ± 6.0 | 41.4 ± 4.0 |
| Urine K+/urine creat. (mmol/mmol) | 179.4 ± 17.2 | 147.6 ± 11.3 |
| Urine Ca2+/urine creat. (mmol/mmol) | 0.84 ± 0.14 | 0.60 ± 0.12 |
| Urine Mg2+/urine creat. (mmol/mmol) | 3.70 ± 1.13 | 1.83 ± 0.77 |
Prox-1DCTKO mice exhibit a significant hypomagnesemia (p = 0.036) compared to the Prox-1DCTCtrl group. For other metabolic parameters, plasma electrolytes, plasma aldosterone, 11-deoxycorticosterone, corticosterone, and urinary ion excretion, no difference was detected between the genotypes. Values are means ± SEM. n = 11 (plasma electrolytes), n = 13 (plasma Mg2+), n = 7 (aldosterone, 11-deoxycorticosterone, and corticosterone), n = 5 (body weight, food and water intake, urine excretion, urine electrolytes)