Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Dec 22;14:542552. doi: 10.3389/fncel.2020.542552

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2020 Chang, Bian, Xiong, Liu, Wang, Zhou, Zhang and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Overexpressed LINC00320 impeded glioma cell proliferation and MVEC angiogenesis. (A) The expression of LINC00320, NFKB1, or AQP9 in glioma cells transfected with oe-LINC00320, oe-NFKB1, or oe-AQP9 detected using RT-qPCR normalized to β-actin. (B) The growth of cells transfected with oe-LINC00320 measured using CCK-8 assay. (C) The cellular tube formation in MVECs upon co-culture with glioma cells transfected with oe-LINC00320 (100×; scale bar = 10 μm). (D) Expression of angiogenesis-related VEGF protein in MVECs upon co-culture with glioma cells transfected with oe-LINC00320 normalized to GAPDH measured by Western blot analysis. (E) Expression of angiogenesis-related CD31 protein in MVECs upon co-culture with glioma cells transfected with oe-LINC00320 normalized to GAPDH measured by Western blot analysis. (F) Expression of angiogenesis-related MMP-2 protein in MVECs upon co-culture with glioma cells transfected with oe-LINC00320 normalized to GAPDH measured by Western blot analysis. (G) Expression of angiogenesis-related MMP-9 protein in MVECs upon co-culture with glioma cells transfected with oe-LINC00320 normalized to GAPDH measured by Western blot analysis. *p < 0.05 vs. cells transfected with oe-NC. Data were expressed as mean ± standard deviation and data between two groups were analyzed by independent-sample t test. Data between two groups at different time points were compared by repeated-measures ANOVA. The experiment was repeated three times independently.