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. 2021 Jan 4;12:81. doi: 10.1038/s41467-020-20439-y

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Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Nasal cavity, c, d tonsil, e, f trachea and g, h lung were collected at euthanasia timepoints (days 3, 5, 7, 14, 20 and 21 pc) for all groups. n = 6 ferrets per group (n = 2 ferrets in the naive sentinel group), with one ferret from each group culled at each timepoint (except day 20 and 21 pc where n = 2 ferrets from the naive sentinel and high dose groups were culled respectively). Viral genomic RNA (a, c, e and f) and viral subgenomic RNA (b, d, f and h) was quantified by RT-qPCR. Bars show values for individual animals. The dashed horizontal lines show the lower limit of quantification (LLOQ) and the lower limit of detection (LLOD). *LLOD range for tonsil represents undetected samples homogenised in smaller volume.