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. 2021 Jan 4;12:81. doi: 10.1038/s41467-020-20439-y

Fig. 5. Re-challenge of ferrets with SARS-CoV-2.

Fig. 5

a Nasal washes were collected at days 1–5 post re-challenge (days 29–33 post-original challenge). Viral RNA was quantified by RT-qPCR. b Nasal cavity, tonsil, trachea and colon were collected at euthanasia timepoints (days 5 and 8 post re-challenge) for all groups. Viral RNA was quantified by RT-qPCR. Bars show values for individual animals. The dashed horizontal lines show the lower limit of quantification (LLOQ) and the lower limit of detection (LLOD). c Percentage weight change from baseline. Baseline was calculated as average of the two most recent weights taken preceding re-challenge. n = 2 ferrets per group with numbers decreasing by 1 at 5 dpc. d Medium dose re-challenged ferret at day 5 post re-challenge. No remarkable changes in alveoli or terminal bronchiole. e Low dose re-challenged ferret at day 5 post re-challenge. No remarkable changes in alveoli or bronchiole. f Control group ferret challenged for the first time (day 28 pc). Inflammatory infiltration within bronchiolar lumen and mild infiltration of alveolar septa; lesions comparable with those observed in the original high dose group. Bar = 50 μm.