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. 2021 Jan 4;4:20. doi: 10.1038/s42003-020-01557-1

Fig. 6. Contribution of the autophagy pathway to protecting OLs from metabolic injury.

Fig. 6

a–c Protective effects of autophagy in human adult OLs. Cell death measured by % PI+ cells of human adult OLs cultured in optimal culture media (N1), in low glucose media (LG) and in combined treatment with chloroquine (N1 + CQ; LG + CQ) after two (a), four (b) and six days (c). Addition of CQ results in increase % PI+ cells at day 4 and 6. N = 5 independent biological samples. d–f Representative western blot for the combined treatment of N1, LG, and NG with and without chloroquine after two days (N = 3 independent biological samples) (d) and quantification of the relative expression compared to actin of LC3 II (e) and p62 (f). Mean ± SEM for each condition in the figure. Statistical significance was verified by ANOVA/Tukey test: *(<0.05), **(<0.01), ***(<0.001).