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. 2020 Oct 7;124(1):281–289. doi: 10.1038/s41416-020-01095-x

Fig. 3. TS expression correlates with EMT gene signature.

Fig. 3

Gene set enrichment analysis of RNA-seq data of a Calu-1 cell sorted for high and low TYMS promoter activity and b A549 cells after transduction of shTS and scrambled pLKO backbone as control. c q-RT-PCR quantification of gene signature from A549 cells. p-value represents multiple t-Test. d Validation of TS-mediated downregulation of indicated genes at protein levels in A549 cells with TS knock-down. e Survival analysis of NSCLC patients from data set GSE101929 based on a knock-down score generated from A549 gene signature in b. p-value is represented as Log-rank (Mantel-Cox) test. f Gene set enrichment analysis with overlapping of TS knock-down score with EMT-signature in SCC patients from data set GSE4573. g A549 cells transiently transfected with 50 nM siRNA targeting transcription factors HOXC6 and HMGA2 identified from a.