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. 2021 Jan 4;12:28. doi: 10.1038/s41467-020-20194-0

Fig. 5. Loss of the orthologous K42, R43, and K115 residues in Drosophila Dichaete (SOXB 2-1) impairs nuclear localization.

Fig. 5

a HA-tagged Dichaete exhibits predominately nuclear localization when expressed in salivary gland cells, and b in salivary duct cells. In contrast, the Dichaete3xMut allele is distributed between nucleus and cytoplasm in salivary gland cells, and is strongly cytoplasmic in salivary duct cells. Scale bar = 50 µm. Digitized images were quantitated to determine the nuclear (Fn) to cytoplasmic (Fc) florescence ratio (Fn/c) as per the legend to Fig. 4, and results are presented as the mean Fn/c ± SEM (n = 62 and 51 (salivary gland), or 52 and 20 (salivary duct) independent cells for the HA-Dichaete and HA-Dichaete3xMut samples respectively). P value determined by using a two-tailed unpaired Welch’s T test in Graphpad prism 8 software; no adjustments were made for multiple comparisons. The precise P value could not be generated, but less <0.000000000000001 and displayed as <0.0001. See also Supplementary Fig. 8 for distribution of data points used to generate mean and SEM values.