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. 2021 Jan 4;4:19. doi: 10.1038/s42003-020-01490-3

Fig. 3. Biophysical characterisation of DOPAL-induced stable Aβ40 oligomers (Aβ-DOs).

Fig. 3

a SDS-page analysis of the pellet fraction of Aβ40 after 20 h of incubation with increasing concentrations of DOPAL. b–d Morphologies and stability measurement of Aβ40-noDOs and Aβ-DOs. b Representative 3D morphology maps measured by AFM of preformed Aβ40-noDOs and Aβ-DOs, with their cross-sectional height and roundness distributions as collected from a. c ThT-based stability measurements of Aβ40-noDOs and Aβ-DOs over the course of 40 h. d TEM images of the samples in b, after 40 h of incubation at 37 °C. Note the distinct fibrillised samples of Aβ40-noDOs after the stability measurement, which is otherwise absent in the Aβ40-DOs samples. For figures b, c and d, assays were performed using the sample containing 10 molar equivalents of DOPAL.