Fig. 4. ¹H-MRS and hyperpolarized [1-¹³C]-alanine can inform on TERT expression or the ALT pathway in isogenic patient-derived low-grade glioma models.

a ¹H-MRS-detectable metabolite levels in MGG119 ALT+ (magenta circles) and MGG119 TERT+ (red circles) neurospheres. b ¹H-MRS-detectable metabolite levels in BT142 ALT+ (magenta circles) and BT142 TERT+ (red circles) neurospheres. c Representative summed ¹³C-MRS spectra showing hyperpolarized [1-¹³C]-alanine metabolism in MGG119 ALT+ and MGG119 TERT+ neurospheres. d Quantification of the ratios for hyperpolarized pyruvate/alanine and hyperpolarized lactate/alanine in MGG119 ALT+ (magenta circles) and MGG119 TERT+ (red circles) neurospheres. e Representative summed ¹³C-MRS spectra showing hyperpolarized [1-¹³C]-alanine metabolism in BT142 ALT+ and BT142 TERT+ neurospheres. f Quantification of the ratios for hyperpolarized pyruvate/alanine and hyperpolarized lactate/alanine in BT142 ALT+ (magenta circles) and BT142 TERT+ (magenta circles) neurospheres. All experiments were performed on three biological replicates (n = 3). Results are presented as mean ± standard deviation. Statistical significance was assessed using an unpaired Student’s t test assuming unequal variance with p < 0.05 considered significant. Correction for multiple comparisons was performed using the Holm–Šídák method. *** represents statistical significance with p < 0.005; ** represents statistical significance with p < 0.01; * represents statistical significance with p < 0.05. Also, refer to Supplementary Fig. 4. Source data with exact p values are provided as a source data file.