Fig. 6. Direct interaction between Kaem and TUFM promotes a protein-protein interaction related to autophagic phagophore formation.

a HepG2 cells were treated with erlotinib or Kaem for 24 h. Co-immunoprecipitation (Co-IP) was conducted using anti-TUFM antibody followed by western blotting. Erlotinib, 20 µM, Kaem, 20 µM. b HeLa cells were treated with erlotinib or Kaem for 24 h. Confocal microscopy was conducted after co-immunostaining with anti-ATG12 and anti-TUFM antibodies. Co-localization analysis of ATG12 and TUFM using ImageJ2 (right). Scale bar, 50 µm. Erlotinib, 20 µM, Kaem, 20 µM.