Fig. 2. Knockdown of USP47 inhibits the proliferation of CML cells.
a USP47 was silenced in K562, K562R, and KBM5T315I cells using a retroviral transduction system as described in Methods. Viable cells were counted in the transfected cell lines at different times (n = 3 biologically independent samples per group). b Cell cycle was measured in the transfected cell lines on day 3 (n = 3 biologically independent samples per group). c Caspase-3 activity was measured after 3 days of retroviral transfection (n = 3 biologically independent samples per group). d Knockdown efficiency of USP47 in normal human BM CD34+ cells was determined by real-time PCR (n = 3 biologically independent samples per group). e, f The number of colonies (e) and cell viability (f) were measured. Data are mean ± s.d. p-values were analyzed by two-way analysis of variance (ANOVA; a, b) or one-way ANOVA (c–f). *p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, no significant. Source data are provided as a Source Data file.