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. 2021 Jan 4;12:51. doi: 10.1038/s41467-020-20259-0

Fig. 3. Usp47 knockout suppresses the development of BCR-ABL-induced CML in mice.

Fig. 3

a Genotyping of Usp47 knockout (Usp47−/−) mice by PCR. b The total BM cells from 8-week-old Usp47+/+ and Usp47−/− mice were counted (n = 6 biologically independent samples per group). Data are mean ± s.d. p-values were analyzed by two-sided Student’s t-test. ns, no significant. c Lin−Sca1+ c-kit+ (LSK) cells from 8-week Usp47+/+ and Usp47−/− mice (n = 3 biologically independent samples per group) were measured by FACS. Data are mean ± s.d. p-values were analyzed by two-sided Student’s t-test. ns no significant. d Survival of mice after receiving BCR-ABL-transduced Usp47+/+ or Usp47−/− BM cells (n = 8 biologically independent samples per group). The experiment was repeated three times. ****p < 0.0001, p-value was analyzed by Mantel-Cox-log-rank test. e The size and weight of the spleen in the two groups of mice were shown on day 45 (n = 6 biologically independent samples per group). center lines of the box and whisker plot represent median values, whereas the box edges indicate the 25th and 75th centiles, and the whiskers indicate the minimum and maximum values. f 35 days after transplantation, the percentages of GFP+ and Gr-1+ cells in BM were examined by FACS analysis (upper panel). The morphology of the cells from PB was examined by Wright-Giemsa staining (lower panel). Scale bar, 20 μm. g H&E staining of liver and spleen from the two groups of mice 45 days after transplantation. Scale bars, 200 × , 100 μm; 400 × , 50 μm. h Immunohistochemical staining of liver and spleen with GFP antibody 45 days after transplantation. Scale bars, 200 × , 100 μm; 400 × , 50 μm. i FACS analysis of the GFP+ LSK cells in BM 35 days after transplantation (n = 3 biologically independent samples per group). Data are mean ± s.d. p-values were analyzed by two-sided Student’s t-test. **p < 0.01. Source data are provided as a Source Data file.