Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Jan 4;12:56. doi: 10.1038/s41467-020-20255-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 4 — a RNAscope staining for Vav2 in intestine from Vil-CreER^T2 Apc^fl/fl (APC) and Vil-CreER^T2 Apc^fl/fl Tiam1^−/− (APC T) intestines. Scale bar represents 100 μm. b Quantification of Vav2 RNAscope in Vil-CreER^T2 Apc^fl/fl (APC) and Vil-CreER^T2 Apc^fl/fl Tiam1^−/− (APC T) intestines. N = 5 biologically independent animals for each genotype *p = 0.0159 as determined by a two-tailed Mann–Whitney test. Data are presented as mean values ±SD. c Images for H&E and BrdU incorporation and RNAscope for Lgr5 and Olfm4 on wild-type (WT), Vav2^−/−, Vav3^−/ Tiam1^−/− (V2V3T), Vil-CreER^T2 Apc^fl/fl (APC) and Vil-CreER^T2 Apc^fl/fl, Vav2^−/− and Vav3^−/−Tiam1^−/− (APC V2V3T). Scale bar represents 100 μm for H&E and BrdU and 50 μm for RNAscope images. d Quantification of BrdU positive cells. N = 7, 6 and 8 biologically independent animals for wild-type, Vil-CreER^T2 Apc^fl/fl (APC) and Vil-CreER^T2 Apc^fl/fl, Vav2^−/− and Vav3^−/− Tiam1^−/− (APC V2V3T) respectively. WT vs APC ***p = 0.0006, APC vs APC V2V3T *p = 0.0013 as determined by a one-tailed Mann–Whitney test. Control data (APC) as in Fig. 3c. Data are presented as mean values ±SD. e Quantification of clonogenicity assay of intestinal organoids. N = 6 biologically independent cell lines for both Vil-CreER^T2 Apc^fl/fl (APC) and Vil-CreER^T2 Apc^fl/fl, Vav2^−/−, Vav3^−/− Tiam1^−/− (APC V2V3T). *p = 0.0260 as determined by a two-tailed Mann–Whitney test. f Compared to Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl (Lgr5 APC), Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl Vav2^−/−, Vav3^−/− Tiam1^−/− (Lgr5 APC V2V3T) mice have a significant survival advantage. N = 19 and 15 biologically independent animals for Lgr5 APC and Lgr5 APC V2V3T respectively. **p = 0.0091 as determined by a two-tailed Log-rank (Mantel-Cox) test. Lgr5 APC control cohort is the same cohort as used in Fig. 3d, e as well as Supplementary Fig. 6D. Data are presented as mean values ±SD. g Quantification of intestinal tumour burden at clinical endpoint following APC loss in the Lgr5 stem cell compartment. N = 8 and 9 biologically independent animals for Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl (Lgr5 APC) and Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl Vav2^−/−, Vav3^−/− Tiam1^−/− (Lgr5 APC V2V3T) respectively. p = 0.9522 as determined by a two-tailed Mann–Whitney test. Data are presented as mean values ±SD. h Solid adenomas (asterisk) were observed in the Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl (Lgr5 APC) cohort, whereas cystic adenomas were observed (H&E) in Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl Vav2^−/−, Vav3^−/− Tiam1^−/− (Lgr5 APC V2V3T) and Lgr5-EGFP-IRES-creER^T2 Apc^fl/fl, Rac1^fl/fl (Lgr5 APC Rac1), as indicated by arrows. Scale bar represents 100 μm. i Intestinal tumour number in an AOM-DSS colitis-associated model of tumourigenesis. Study was carried out on wild-type mice or Vav2^−/−, Vav3^−/− Tiam1^−/− mice. N = 9 and 4 biologically independent animals for wild-type or Vav2^−/−, Vav3^−/− Tiam1^−/− respectively. **p = 0.0042 as determined by a one-tailed Mann–Whitney Test. Data are presented as mean values ±SD.