Table 1.

Purification chart of CmSP (for 100 g of seed flour)

Purification step	Total Protein (mg)	Protease activity (Units)a	Specific Activity (U/mg)	Fold purification	Activity recovery (%)
Crude extract	2935	95,400	32.5	1	100
Ammonium sulfate fractionation (30–70%)	1591	79,250	49.8	1.5	83.1
DEAE Sephadex A-50	196	42,500	216.8	6.6	44.5
Sephadex G-50	39	34,500	884.6	27.2	36.2

^aUnit: One unit of the protease activity is defined as the amount of enzyme required to cause an increase in 0.01 unit of absorbance at 660 nm per minute at 37 °C