Fig. 5.

Expression levels of pigments biosynthetic genes in proposed biosynthetic pathway of MPs. The expression levels of 13 genes on the 3rd, 5th, and 7th day of fermentation of the control and AN treatment samples were analyzed by RT-qPCR. The control samples were 1 (3 days), 3 (5 days), 5 (7 days), and the AN samples were 2 (3 days), 4 (5 days), 6 (7 days). The transcriptional levels were normalized to those of the actin gene. The mRNA levels on the 3rd day of control were used as the reference value. The assay was repeated by three times