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. 2021 Jan 4;6:2. doi: 10.1038/s41536-020-00113-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Autologous dermal fibroblast sheets (DFSs) can be harvested by low-temperature treatment and are harvested as intact sheets. Hematoxylin and eosin (A) and Azan (B) staining show that DFSs are composed of two to seven cell layers. Azan staining also showed extracellular matrix present within the harvested DFSs. Immunohistological analysis is shown for expression of vimentin (C), alpha-smooth muscle actin (D), collagen type 1 (E), collagen type 3 (F), and fibroblast growth factor 2 (G). Scale bars indicate 100 μm.