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. 2020 Dec 1;124(1):207–216. doi: 10.1038/s41416-020-01173-0

Fig. 3. 4T1 developed a higher number of lung metastases in vivo.

Fig. 3

a Lung metastases count. ***P < 0.001. b Representative H&E staining of lung metastases of BALB/c mice s.c injected with TUBO, 4T1 or TS/A cells and BALB-neuT mice. Amplification ×10. c GLUT1 fluorescence intensity calculated for BALB-neuT, 4T1 and TS/A tumour slice compared to TUBO intensity. ****P < 0.0001. df Cytofluorimetric analysis of 10 mm mean diameter tumours (400 mm3) explanted from BALB-neuT mice or from BALB/c s.c. injected with TUBO, 4T1 or TS/A cells. Graphs showing the mean ± SD of the percentage of Sca1+ or CD44+CD24 cells and CD44 mean fluorescence intensity (MFI) from three independent experiments. *P < 0.05; **P < 0.01; ***P < 0.001.