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. 2020 Nov 4;124(1):217–227. doi: 10.1038/s41416-020-01094-y

Fig. 2. Phospho-PR promotes tamoxifen insensitivity in ER+ breast cancer models.

Fig. 2

a T-47D and MCF-7 wt PR-B and PRB-K388R cell growth in tamoxifen as measured by manual counting of the cells after 5 days in culture. Cell number was normalised to day 0. b Protein and c mRNA expression of the IGF1-R system. d Colony formation of T-47D and MCF-7 cells expressing empty vector (EV), wt PR-B, phospho-mutant PR-B (PRB-S294A), or hyperactive PR-B (PRB-K388R) grown in insulin-containing soft agar. e Soft agar colony formation of wt PR-B and PRB-K388R cells grown in the presence of insulin (10 nM) and tamoxifen (Tam; 100 nM). Error bars are S.E.M., n = 3 biological replicates; *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.