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. 2020 Nov 4;124(1):217–227. doi: 10.1038/s41416-020-01094-y

Fig. 4. IRS-1 inhibition decreases tumoursphere formation.

Fig. 4

a ALDH1 activity was determined using the Aldefluor assay in T-47D tumourspheres. b CD44+/CD24− populations in T-47D tumourspheres were analysed by flow cytometry. c The effect of NT157 (IRS-1 inhibitor; 3 µM) and 83-7 monoclonal antibody (insulin receptor degrader; 5 µg/mL) treatment on tumoursphere number and d ALDH1 activity was examined. e Tumoursphere formation was examined after inducible IRS-1 knockdown. Inset confirms Dox-induced knockdown of IRS-1 protein by western blot. Graphed data are mean ± S.E.M. of combined n = 4 independent experiments (technical replicates). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001.